Dna Strawberry Extraction

In: Science

Submitted By archstanton216
Words 375
Pages 2
Extracting DNA from a Strawberry

Preparation/Set-Up

Place ethanol in freezer prior to experiment and allow to stand until cold. Prepare a container for the DNA by wrapping a small tray with saran wrap. Preparing an Extraction Buffer

Fill a container with approximately 900 mL of water Add 50 mL of dish washing soap or shampoo (DO NOT use any shampoo containing conditioner) Add 2 teaspoons of salt Slowly Invert container and allow contents to swirl

- This section will contain a graphic of the type of tube that will hold the buffer

Preparing your Filtration Apparatus

Obtain a funnel and cheesecloth Drape the cheesecloth over the edge of the funnel, securing firmly Place the tube of the funnel into a 50 mL glass vial - A graphic will be inserted showing the completed apparatus

Procedure

Preparing the Strawberries

Obtain 2 to 3 strawberries and remove all stems and leaves Place strawberries in a zip lock bag Seal ziplock bag tightly, allowing no air to remain Use your fingers to squish the strawberries. Continue with this step for two minutes or until strawberries are completely reduced to paste. -Graphic here to illustrate process of squeezing bag

Adding the Buffer

Add approximately 3 tablespoons of the buffer to the zip lock bag and then reseal tightly, being sure to allow no air to remain Use your fingers to squish the mixture, allowing the buffer to distribute evenly for 1 minute Squeeze the mixture out of the bag onto the funnel Wait for approximately 10 minutes, allowing the solution to filter into the cup as completely as possible Discard the remaining strawberry and filter Remove the strawberry/buffer solution and place in a separate vial, removing as much of the paste as possible
Extracting the DNA Tilt the jar slightly, allowing ethanol…...

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