Isolation and Purification of Lyngbya Majuscula on Nutrient-Enriched Agar Plates

In: Science

Submitted By momochan016
Words 2669
Pages 11
Isolation and Purification of Lyngbya majuscula on Nutrient-enriched Agar Plates

A Special Problem

ABSTRACT

Lyngbya majuscula samples were obtained from the Phycology Laboratory stock culture of the UPV Institute of Aquaculture. Five (5) mm fragments of these were inoculated into agar plates that used 1% agar concentration enriched with varying concentrations (1.0%, 1.5%, and 2.0%) of Hughes, et. al. (1958) Mineral Medium No. II.

After ten (10) days of culture, the Trichome Length (TL), Trichome Width (TW), Sheath Width (SW) and Total Length of the Lyngbya filaments were measured from photomicrographs of the samples using Image Tool (Version 3.00) developed by the University of Texas Health Science in San Antonio (UTHSCSA).

Lyngbya filaments in all cultures enriched with the Hughes medium obtained significantly higher TL and TW over those of the control treatment. But enrichment of the agar medium did not result in an increase of sheath widths.

Filaments cultured in 1.5% and 2% enriched agar showed contamination. Those in 1% nutrient concentration had comparable growth, but no contamination. Furthermore, Lyngbya cultured in enriched agar did not grow beyond the area of inoculation. Isolation was also more difficult in agar than in the liquid control medium. INTRODUCTION

As early as the 1900s, Lyngbya sp. blooms were documented in the areas of Eastern Moreton Bay and Bribie Island in Australia (Abal & Lawn, 2004). Lyngbya is a genus which is composed of species which are usually inhabitants of the sea. Numerous species reside in estuarine places and in brackish channels. Others are found in freshwater areas and thermal springs (Harvey, 1857). Lyngbya has been found to be a nuisance in the areas of South Eastern United States. It is a cyanobacteria which lives naturally in the benthic zone (Gross & Martin, 1996). In recent studies,…...

Similar Documents

Water Purification

...------------------------------------------------- Water purification From Wikipedia, the free encyclopedia This article is about large scale, municipal water purification. For portable/emergency water purification, see portable water purification. For industrial water purification, see deionized water. For distilled water, see distilled water. For the treatment of sewage see sewage treatment Water purification is the process of removing undesirable chemicals, biological contaminants, suspended solids and gases from contaminated water. The goal is to produce water fit for a specific purpose. Most water is purified for human consumption (drinking water), but water purification may also be designed for a variety of other purposes, including meeting the requirements of medical, pharmacological, chemical and industrial applications. In general the methods used include physical processes such as filtration,sedimentation, and distillation, biological processes such as slow sand filters or biologically active carbon, chemical processes such as flocculation and chlorination and the use of electromagnetic radiation such as ultraviolet light. The purification process of water may reduce the concentration of particulate matter including suspended particles, parasites, bacteria,algae, viruses, fungi; and a range of dissolved and particulate material derived from the surfaces that water may have made contact with after falling as rain. The standards for drinking water quality are......

Words: 5840 - Pages: 24

Purification of Organic Compounds

...3. INTRODUCTION Organic compounds are isolated either from natural sources or from reactions mixtures. These compounds are seldom pure and are usually contaminated with small amounts of other similar compounds, which are found to exist together or formed during the reaction. In order to characterize them, it is important to purify them. 4. PROCESSES FOR PURIFICATION 1. Crystallization 2. Sublimation 3. Distillation 4. Chromatography 5. Differential Extraction 6. Qualitative Analysis 5. 1. CRYSTALLIZATION Aim To separate a solid compound in pure and geometrical form. Principle A saturated solution of the impure substance in a suitable solvent is made at a temperature higher than the room temperature. On cooling this solution, the substance reappears in the form of well shaped crystals. Process Purification by crystallization involves the following steps: Choice of solvent Preparation of solution Filtration of the solution Separating the crystals Drying of crystals Example Crystallisation of Phthalic acid 6. 2. SUBLIMATION Aim To separate volatile solids, which pass directly into vapour state on heating from a non-volatile solid. Principle A mixture of solid substances, such as camphor, benzoic acid, ammonium chloride, iodine etc., containing non-volatile substances, when heated, change directly into vapour without passing through the liquid state. Process 7. Fig :-Sublimation 8. 3. DISTILLATION Aim To separate a solution of a solid in a liquid and for separating a......

Words: 853 - Pages: 4

Water Purification

...Water and water purification A plentiful supply of suitable water is essential for a dyeing and bleaching plant. Before modern methods of water purification were purification were available, the textile industries tended to congregate in areas where the natural water supply was plentiful and sufficiently pure. Water with a high degree of purity is rarely obtainable from natural sources. A knowledge of he impurities which will probably be present and how to remove them is, therefore, desirable. Supplies of water may be classified as follows: i) rain water ii) surface water, which consists of rain which has collected into streams, rivers, or lakes; iii) subsoil water, which has percolated a small distance into the ground. It is collected from shallow wells or surface springs; and iv) deep well waters, which have usually percolated through several strata. ➢ Rain water:- Rain, collected immediately after propitiation, is the purest of all natural waters. It may contain traces of gases dissolved out of the atmosphere, and possibly an almost infinitely small amount of finely divided solid matter derived from the air. In the neighborhood of towns rain may collect dissolved or suspended impurities such as soot, traces of sulphur dioxide, or sulphuric acid, and other by-products of industrialization. Rain water from such sources is improved by filtration through a bed of sand. Although the collection of rain for industrial purposes dose not......

Words: 2188 - Pages: 9

Isolation and Process Optimization of Textile Dye Degrading Bacteria

...Title: Isolation and Process Optimization of textile dye degrading bacteria Abstract: Bacteria can remove dyes from wastewater and soil, leading to a decrease in their toxicity. The detoxification rate depends upon media and culture conditions. The decolourization/removal of textile dyes like bromophenol blue, methylene blue, methyl green, and malachite green, in addition to various other industrial dyes, using bacteria isolated from soil has been an important area of research for bioremediation and the present work is focused on this specific aspect. The effect of independent variables such as time, temperature, pH, and agitation on decolorization efficiency of bacteria has been optimized. Biodegradation of methylene blue and bromophenol blue was demonstrated by monitoring the effects of the different parameters and determining optimal conditions for degradation activity.   Introduction Several industrial activities, such as textile dyeing, olive oil extraction and the manufacture of pulp and paper are characterized by intensive water consumption rates. They release huge amounts of more or less coloured effluents into the environment. As far as synthetic dye release is concerned, textile dyeing facilities and the manufacture of dyestuffs are two major polluting sources. In addition, traditional textile dyeing processes generate a large amount of coloured effluents, because about 100 litres of water are required to process 1 kg of dyed fabrics. Moreover, up to 15% of......

Words: 6765 - Pages: 28

Isolation, Identification of Microbes Isolated from Thar Desert

...ISOLATION,IDENTIFICATION AND CHARACTERIZATION AND APPLICATIONS OF MICROBES ISOLATED FROM THAR DESERTSOIL,INDIA INTRODUCTION This study was done to identify the characterization, isolation and identification of microorganism from the desert soil. In the present study, thermal resistant and osmotolerant bacteria from the Thar soils were reported. Bacteria were isolated from 20 soil samples of 100gm each, collected from sand dunes. As Thar Desert were also evaluated for their plant growth promontory effects in terms of seed germination, shoot and root length elongation and total biomass production. It also tells about the occurrence of nodulated legumes. Most of the bacteria grow best in normal environmental conditions, but some grow in extreme conditions like extremes of pH, temperature, high salt reservoirs of microorganisms, as bacteria, protozoans, yeasts, molds, and microscopic worms are routinely found in this environment and are continuously submitted to environmental and anthropogenic perturbations which can lead to serious modifications of microbial taxonomic and functional diversity present in desert soil, therefore some of the fungi like Thermophilic fungi are also present in these conditions. Moreover DNA Based Identification and Characterization of microorganisms were also done from the soil of Thar Desert. Along with this, application of the microorganism isolated from the thar desert are like in biofertiliser, for medicinal parouses etc., example Ricinus......

Words: 800 - Pages: 4

Nutrients

...life cycle, spanning from age 20 to 50. Vitamins and minerals are an important part of nutrition. Vitamins are organic substances present in food. They are required by the body in small amounts to regulate metabolism and to maintain normal growth and functioning. Minerals are vital because they are the building blocks that make up the muscles, tissues, and bones. They also are important to many life-supporting systems, such as hormones, transport of oxygen, and enzyme systems. Oat bran, which is a whole grain, can supply fiber and mineral called magnesium. Foods high in fiber are usually good to eat as it helps to prevent constipation. Good nutrition can help prevent weight gain by focusing on consuming calories that are high in nutrients, not in sugars and fat. Nutrition is an essential part in preventing and controlling diseases. Poor nutrition can lead to high cholesterol, which causes coronary heart disease. Lowering salt intake can control high blood pressure. High blood pressure runs in my family and I have it as well. I take a pill every morning to regulate it. My goal is to increase my physical activity and alter my eating habits in order to bring it to a healthy level. A proper diet would recommend eating at least three ounces of whole grain bread, cereal, crackers, rice, or pasta every day. At least one-half of all grains should be whole grains. The food pyramid recommends eating more dark green and orange vegetables, as well as more dry beans and......

Words: 698 - Pages: 3

Isolation

...Rachel Murrell Brown girl, Brownstones Isolation is an important issue that the narrator highlights in the novel ‘Brown Girl, Brownstones’. Discuss the importance of isolation in the novel using textual evidence from the novel. One of the themes in the novel ‘Brown Girl, Brownstones’ is the issue of isolation. The narrator skilfully crafts a riveting story about a group of Bajan immigrants in 1939 who make Brooklyn, New York their home. The narrator employs a range of narrative techniques to highlight this important issue, however there is a heavy dependency on setting, language and characterisation which simultaneously bring to the forefront the several forms of isolation encoded in the novel. The narrator details the isolation and plight of the Bajan community who have come to New York. Set against the backdrop of the effects of World War ІІ and the Great Depression, the narrator pays great attention to the Bajan community by observing the inter-relationship of the Boyce family members and the friendship of the Bajan women. The narrator uses setting as a narrative technique in highlighting the important theme of isolation in the novel. She has placed the Boyce family in Brooklyn where they lease a brownstone house. Both the Boyce family and Bajan community altogether live and share close relationships with one another but it seems that the immigrants are secluded from the wider city. The narrator’s description of their community depicts a sense of segregation......

Words: 1431 - Pages: 6

Nutrients

...increases your blood flow and your nutrients to your tissues, and muscle coordination. Body Composition Amount and relative proportions and distributions of fat mass and fat free mass in the body. Saying physically fit, Watching what you eat, and balancing your daily life such as work, school, and health. Helps your mental health, helps yourself esteem. Part 2: Critique a Meal For this part of the assignment, you critique the nutritional value of a meal. You will select this meal from the USDA website. Follow the instructions below: • Access the USDA Recipe Finder search tool at http://recipefinder.nal.usda.gov/ o (either click on or paste the above link in your internet’s address bar) • Search for a recipe using one of the available criteria: o Type in ingredients; o Type in a recipe name, or; o Choose options from the available categories • Select a recipe to critique from the generated results. Review the nutritional information provided for the recipe you selected or added and answer the questions below. 1. What is the name of the recipe you are evaluating? Beef Stroganoff 2. Which essential nutrients—carbohydrates, fats, proteins, vitamins, and minerals—are included in the recipe? 3. Which ingredients are providing each nutrient? 4. Does this recipe include a good balance of essential nutrients? Explain your answer. 5. How could the recipe be altered to include more essential nutrients? 6. Do you......

Words: 614 - Pages: 3

Isolation

...29, 2015 Essay 2 Modernism/Post-Modernism World Lit Isolation Various radical and utopian influences stimulated by new ideas in psychology, philosophy, psychoanalysis, and political theory were influential to various modernist works. Franz Kafka's, “The Metamorphosis”, which was written during the era of Modernism, provides dynamic visuals meant to awaken its readers to the frightening isolation of individuals in the modern, bureaucratic state, as well as providing an autobiographical undertone. Moreover, the work is reportedly one of the few stories that satisfied him enough that he desired that it be published. In order to truly appreciate Kafka's work it is important to understand who he was and what his state of mind and overall disposition was when he wrote “The Metamorphosis”. In many regards his own personal background mirrors the character Gregor in the story. Although Kafka was intelligent, he lacked confidence and assertiveness in his daily interactions with others. It could be speculated that he felt like an outcast being a German-speaking Jew living in Prague. Throughout the story his inadequacy is represented by the theme of isolation. The insect that Gregor has transformed into following some unpleasant dreams could signify his self-concept, but also his desire to convey the target audience's sense of isolation in the modern, bureaucratic state. There are several themes of isolation that occur during the story. Gregor feels isolated from......

Words: 927 - Pages: 4

Nutrients

...Nutrients All differents types of nutrients:- Carbohydrates, Protein, Fat, Folacin, Niacin, Calcium, Iron, Zinc, Fluoride, Chloride, Chromium, Copper, Iodine, Magnesium, Manganese, Molybedenum, Phosphorus, Potassium, Selenium, Sodium, Pantothenic Acid, Biotin, Vitamin A, Vitamin B1, Vitamin B2, Vitamin B6, Vitamin B12, Vitamin C, Vitamin D, Vitamin E, Vitamin K. Functions, Symptoms and Sources. Protein The function of Protein is building up tissue proteins, helps maintain fluid balance, it is a source of energy, helps in the formation of anti-bodies, maintenance of acid-base balance and an important part of enzymes and hormones. If your body has not taken the appropriate amount of protein it will experience Deficiency Symptoms such as Kwashiorkor-edema, reddish pigmentation of hair and skin, fatty liver, retardation of growth in children, diarrhea, dermatosis, decreased T-cell lymphocytes with increased secondary infections. You can obtain protein through food sources such as breast milk, infant formula, meat, fish, poultry, egg yolk, cheese, yogurt, legumes. Carbohydrate The function of Carbohydrates is protein sparing, it is the major energy source, also it is necessary for normal fat metabolism, glucose is the sole source of energy for the brain, many sources also provide dietary fiber. Lack of carbohydrates will result to Ketosis. Food sources of Carbohydrates are breast milk, infant formula, whole-grain breads, cereals, and other fortified or enriched grain......

Words: 511 - Pages: 3

Lab Isolation

...cultures of specific organisms. Procedures Exercise 1 Part I 1. Disinfect the work area. 2. Melt the agar tubes. 3. Leave the 18 mL tube of MRS agar in hot water (50°C) for use in Part II. 4. Use the marking pencil to label the bottom of one Petri dish S. epidermidis. Pour one half (9mL) of the contents of a tube of nutrient agar into the S. epidermidis Petri dish and the other half into the bottom of an unmarked Petri dish. Cover the dishes and allow them to solidify for use in Part IV. 5. Pour the remaining melted nutrient agar into the unmarked Petri dishes (half a tube per dish). Cover the dishes and allow them to solidify for use in Part III. Part II 1. Disinfect the work area. 2. Label the bottom surface of three sterile Petri dishes L. acidophilus #1, #2, and #3, respectively. 3. Disinfect three test tubes by submerging them in boiling water for 5 minutes. The tubes will be hot, so use tongs or tweezers to lift them out of the water. Be careful not to contaminate the tubes by touching their lips or interiors. When the tubes are cool, label them to match the L. acidophilus Petri dishes. 4. Divide the liquid MRS agar into the three test tubes marked L. acidophilus. If the agar has begun to solidify, reheat it until it is fully melted. Set the test tubes of agar in the hot water to prevent them from solidifying. 5. After ensuring the tubes of agar are cool enough not to kill the bacterial culture but are still fully liquid, use aseptic......

Words: 1233 - Pages: 5

Isolation and Characterization of Endophytes from Indegineous Tree Legumes

... Isolation and characterization of bacterial endophytes from indigenous tree legumes (Colophospermum mopane) and screening for their potential to produce plant growth hormones. HIT 400 Project Proposal submitted in partial fulfillment of the Requirements for the award of the degree of Bachelor of Technology (Hons) Degree in Biotechnology To Biotechnology Department School of Industrial Sciences and Technology Harare Institute of Technology (HIT) By Edinah Tembo` H1210549T Under the supervision of Mrs P.D Chiunzi 1.0 Introduction The status quo of Zimbabwe’s agriculture seeks to increase productivity along with a long term maintenance of a sustained yield. This aim however, can only be successfully achieved if there is a sufficient supply of plant growth hormones to the plants. Due to the market globalization, the exporting of the agricultural products is rapidly rising hence a need to meet the export demand. Zimbabwe has an abundant variety of indigenous tree legumes, unfortunately scientific taxonomic knowledge of the inhabiting endophytic species is limited and more has to be done to catch up with the rest of the world . The performance of the Zimbabwean economy has always been historically dependent and anchored on the production of the primary sector that is mining and agriculture. Although unpredictable rains continue to adversely affect the sector, the 2000 land reform programme made significant strides in employment creation and improving the livelihoods of......

Words: 3054 - Pages: 13

Nutrient Analysis

...A large part of an individual’s day-to-day life is both eating and performing physical activity. The Choose My Plate/ Super Tracker website provided by the U.S. government provides a way to plan, track, and compare meals based on the nutrients needed by the individual. The website also provides an eating plan based on your height, weight, level of physical activity, and diet goals. Additionally, the website produces a nutrient report detailing the individuals average intake of a variety of nutrients, and compares and contrasts it to the target amount. Consuming proper nutrition is extremely important in living a healthy lifestyle. Paying clear attention and keeping an eye on your personal daily nutrition values and intake is vital to maintaining an adequate diet. The purpose of this paper is to analyze my personal dietary intake as a way of better understanding the ways in which the foods I consume are helping or hurting my overall nutrition and health. My personal daily intake will be analyzed to determine what foods are providing the right type of nutrients, and how food consumption might be adjusted to provide for a healthier nutrient intake. For this analysis, I created a plan using Super Tracker that was based on my current weight and activity level, and my goal to lose weight. My physical activity was tracked for the week of March 13- March 19, and my meals were tracked for the day of March 15, 2016. Prior to this project, I had recently changed my lifestyle. My eating...

Words: 299 - Pages: 2

Isolation of Microorganisms from Evironmental Samples

...University of Santo Tomas College of Science Isolation of Microorganisms from Environmental Samples Experiment 6 (Group 6) JALIPA, Jomari T. JAVIER, Rhea A. JIMENEZ, Ma. Mikaela P. JURILLA, Joaquim Gerardo L. March 2016 ABSTRACT The exercise aims to enumerate the various microbial species that are present in a particular environment by collecting different substances such as pond water and soil that will be the source of microorganisms to be isolated and identified. Moreover, this exercise also aims to isolate microorganisms from the environment. This will be done by exposing half-strength Nutrient Agar plates to a specific site the experimenters chose. After which, the Agar plate will be incubated and the colonies formed were observed in the laboratory. INTRODUCTION There are approximately 10,000 named species of microorganisms. It is estimated that there are between 10,000 and 100,000 more unidentified species for every identified one (Truckee Meadows Community College, n.d.). Upon appearance, these microbes have evolved, diversified and virtually occupied every habitat possible on earth; from extreme environments to the corners of our nails, or from the deepest parts of the ocean to the tip of the high mountains. The ubiquity of these microorganisms causes them to populate in natural environments whether suspended in air, in water or associated with other organisms (ie: humans). Different surfaces whether inside or outside...

Words: 1518 - Pages: 7

Isolation

...Lastly, droplet (influenza) and airborne (tuberculosis) precautions are initiated when infection is probable through respiration (Siegel et al., 2007). While droplet precautions are much more extensive than contact precautions, both require the patient to be isolated in a separate room with use of all PPE. Isolation precautions pose a logistical and safety problem to the clinic, as well as posing a risk to the patient’s psychological health, and his/her right to consistent and thorough medical care. The clinic has the task of identifying those who should be placed on isolation precautions, which can be inefficient due to culture testing times, which can yield results up to 24 hours after culturing. Another problem is the lack of accountability of the patients, their family, and caregiver’s activities when not at the clinic. Long wait times force the patients to be interspersed in the waiting rooms with access to shared materials, such as magazines and books. Once in the clinic, the isolation precautions are not always instituted due to miscommunication, lack of space, or lack of appropriate supplies. It was documented by many studies that if patients were placed in an isolation room with the proper signage, compliance was still low. Nurses felt the time requirement to put on the proper PPE was too extensive, and the masks and goggles were causing a problem with vision. These obstacles compromised the time...

Words: 582 - Pages: 3

124 komentarze | Descargar Office Professional ..ISO | Frosted Window Tint Etch Privacy Film Frost Self Adhesive Sticky Back Plastic